The Vole Sex Hormone ELISA Kit is available in stock. Shanghai Jinma Biotech Co., Ltd. specializes in providing high-quality ELISA kits for various research applications. When you purchase our ELISA kit, you can also enjoy free detection services. Recently, a student from Sichuan University contacted us, mentioning that there were some issues with the ELISA kit they ordered. They hope we can provide some technical support and guidance to help them better understand the process.
When performing experiments using the Vole Sex Hormone ELISA Kit, it's important to be aware of potential experimental errors. Here are the key steps recommended by Shanghai Jinma Technology to ensure accurate results:
1. Make sure to shake all reagents thoroughly before use. Avoid creating excessive foam, as this can lead to bubble formation during sample loading, which may cause measurement inaccuracies.
2. Determine the number of plates required based on the number of samples and standards. It’s recommended to use duplicate wells for each standard and blank control. Each sample should be tested according to its specific quantity. For example, if possible, use duplicate wells to improve reliability.
3. Add 50 µl of the diluted standard and 50 µl of the sample into each reaction well. Immediately add 50 µl of biotinylated antibody, cover the plate, gently mix, and incubate at 37°C for 1 hour.
4. After incubation, remove the liquid from the wells and add washing buffer. Shake for 30 seconds, then remove the buffer and pat dry with absorbent paper. Repeat this washing process three times. If using an automated washer, increase the number of washes by one.
5. Add 80 µl of streptavidin-HRP conjugate to each well, mix gently, and incubate at 37°C for 30 minutes.
6. Remove the liquid again, fill the wells with washing buffer, shake for 30 seconds, remove the buffer, and pat dry. Repeat this washing step three times. If using a washer, increase the number of washes by one.
7. Add 50 µl of substrate A and 50 µl of substrate B to each well, mix gently, and incubate at 37°C for 10 minutes. Be sure to avoid exposure to light during this time.
8. Once the incubation is complete, quickly add 50 µl of stop solution to each well and measure the results immediately after adding the stop solution.
9. Finally, measure the optical density (OD) of each well at 450 nm to obtain the final results.
Our company is committed to applying advanced international technologies and scientific knowledge in the development and production of ELISA test kits. We take pride in delivering products that offer high reliability, sensitivity, repeatability, and specificity. This ensures consistent and accurate experimental results, making our kits a preferred choice among researchers and laboratories worldwide. With our quality assurance, we continue to gain trust and repeat business from satisfied customers.
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